Cov khoom siv CE tsim nyog no, siv cov thev naus laus zis ntau yam fluorescence tshawb pom, ua kom muaj qhov tseeb ntawm cov kab mob hauv cov hlab ntshav thiab lawv cov tshuaj tseem ceeb ntawm cov noob hauv ib qho kev soj ntsuam, nrog rau kev tshawb pom ntau dua thiab raug. Ua ke nrog lub tshuab hluav taws xob digital PCR tag nrho, tag nrho cov haujlwm ua haujlwm los ntawm cov qauv mus rau kev tshaj tawm tuaj yeem ua tiav hauv 3 teev, txo cov hnub ib txwm muaj - tos ntev cuam tshuam nrog ntshav kab lis kev cai thiab muab kev txiav txim siab tseem ceeb- ua kev txhawb nqa rau kev kho mob thaum ntxov - kev kho mob.

Kev Ntsuas Scope

(I) Kev Tshawb Fawb Lub Hom Phiaj (Pib faib ua 4 pawg)

Vaj huam sib luag	Hom phiaj
Ib	Pseudomonas aeruginosa Klebsiella pneumoniae Escherichia coli Acinetobacter baumannii
Ob	Staphylococcus aureus Enterococcus + Candida * Streptococcus +
Peb	Stenotrophomonas maltophilia Cov kab mob enterobacter cloacae Proteus mirabilis Coagulase-tsis zooStaphylococci * Serratia marcescens
Plaub	KPC mecA OXA-48 NDM/IMP vanA/vanB

Lus ceeb toom:

Enterococcus:Enterococcus faecalis, Enterococcus faecium
Candida:Candida albicans, Candida glabrata, Candida parapsilosis, Candida tropicalis, Candida krusei
Streptococcus:Streptococcus pneumoniae, Streptococcus anginosus, Streptococcus pyogenes, Streptococcus mitis thiab Streptococcus agalactiae
Coagulase -negative staphylococci:Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus cephalae, Staphylococcus haemolyticus, Staphylococcus lugdunensis, Staphylococcus warneri

(II) Cov qauv siv tau

2-5 mL Peripheral ntshav

Clinical Application Scenarios

01/

Cov txiaj ntsig hauv 3 teev

02/

Kev tshuaj xyuas thawj zaug rau BSI

03/

Kev ceeb toom ntxov rau sepsis

04/

Pab kuaj mob

05/

Dynamic saib xyuas

06/

Tsis muaj ntshav kab lis kev cai.

Core Background ntawm Sepsis

1.Definition: Infection + SOFA Ntau dua los yog sib npaug li 2 , lub neej -kev hem thawj ntawm lub cev tsis ua haujlwm tshwm sim los ntawm tus tswv tsev dysregulated teb rau tus kab mob, uas yog kab mob kis tau los ntawm cov kab mob xws li kab mob thiab cov kab mob nkag mus rau hauv cov hlab ntsha.
2.Core Challenges:

Kev tshwm sim siab: Qhov tshwm sim hauv cov teb chaws tsim kho yog 437 rau 100,000 tus neeg, 49 lab hauv 2017 WHO cov ntaub ntawv.
Kev tuag siab: Cov neeg tuag muaj li ntawm 20% mus rau 50%, ua rau muaj txog 11 lab tus neeg tuag txhua xyoo (WHO, 2017).
Kev kub ceev rau kev kho mob: Rau txhua teev ntawm kev kho mob qeeb, tus neeg tuag tau nce 7.6%; kev ncua 6-teev ua rau muaj 58% nce hauv kev tuag.
Cov nqi kho mob siab: Tus nqi tsev kho mob nruab nrab yog siab txog $ 11,390, nrog rau qhov nruab nrab ntawm $ 502 toj ib hnub.

Cov kev txwv ntawm Kev Tshawb Fawb Cov Ntshav Ntshav

Lub sijhawm hloov pauv ntev:

Siv 2-3 hnub los yog ntau tshaj.

Tsawg tus nqi zoo:

Kwv yees li 10%.

Qhov tshwm sim tsis tseeb:

Muaj peev xwm ua tau tsis raug.

High contamination risk:

Muaj kev pheej hmoo siab ntawm kev kis kab mob thaum lub sijhawm ua haujlwm.

Loj cov ntshav ntim ntim:

20ml-60ml kuaj ntshav hauv ntau.

Kev saib xyuas tsis yooj yim:

Nws tsis tuaj yeem siv rau kev saib xyuas dynamic.

Core Advantages ntawm Digital PCR Detection Kit

1. Ceev thiab ua tau zoo:Nws tsuas yog siv sijhawm 3 teev los ntawm cov qauv mus rau qhov tshwm sim, ua tau raws li qhov xav tau ntawm kev kho mob sai sai.
2.High zoo tus nqi:Tus nqi zoo yog nce tsawg kawg yog 200%.
3. Cov qauv me me yuav tsum muaj:Tsuas yog 2 mL-5 mL ntawm cov ntshav tag nrho yog xav tau.
4.Kev txhawb nqa rau kev saib xyuas dynamic:Nws tuaj yeem soj ntsuam cov kab mob thiab cov tshuaj tiv thaiv kab mob hauv cov neeg mob, coj kev hloov kho raws sij hawm thiab raug ntawm cov tshuaj tiv thaiv kab mob.
5. Yooj yim rau kev khiav lag luam:Nruab nrog Automated Nucleic Acid Detection Reaction Construction System (AP10) thiab Automated Digital PCR System (AD3207), nws muaj qib siab ntawm cov txheej txheem automation.
6.Validation:Nws tau dhau los ua qhov kev sim tshuaj ntsuam xyuas dav dav thiab cov zej zog kho mob tau pom zoo tias peb txoj kev tuaj yeem siv rau kev tshuaj ntsuam xyuas ntxov ntawm kev xav tias sepsis.

1. 3 Sijhawm vs. 3 Hnub: Qhov Tseem Ceeb Qhov Sib Txawv hauv Cov txiaj ntsig kho mob

Sijhawm Dimension	Peb qhov kev daws teeb meem (3 teev)	Txoj Kev Ncaj Ncees ( Ntshav Kab lis kev cai + qPCR, 2-3 Hnub)
Teev 1	Cov qauv sau ua tiav; pib txheej txheem nrhiav pom.	Cov qauv sau thiab muab tso rau hauv cov khoom siv ntshav kab lis kev cai. Nkag mus rau lub sijhawm tos ntawm microbial loj hlob.
Teev 3	Daim ntawv tshaj tawm: Kev txheeb xyuas kab mob, kev thauj khoom kom muaj nuj nqis, thiab cov noob tseem ceeb tiv thaiv (xws li MRSA, ESBL) tau txiav txim siab. Cov kws kho mob tuaj yeem kho kho tam sim nrog qhov tseeb.	Blood culture instrument may signal early positivity (typically >12 teev), tab sis tsis tuaj yeem qhia cov kab mob lossis muab cov ntaub ntawv cuam tshuam. Cov kws kho mob tseem nyob hauv thaj chaw kho qhov muag tsis pom kev.
12-24 teev	Tus neeg mob tau txais ntau tshaj 24 teev ntawm qhov tseeb, cov hom phiaj kho; Cov cim kab mob tuaj yeem qhia tau tias muaj kev txhim kho.	Ntshav kab lis kev cai ua ntej zoo; kev pom zoo cov kauj ruam (smear, subculture) pib (yuav tsum tau ntxiv 12-24 teev).
Saib 48–72	Thawj qhov kev soj ntsuam ua tau zoo tuaj yeem ua tau; Lub hom phiaj kev ntsuas ntawm kev kho cov lus teb los ntawm kev hloov pauv tso cai rau kev ua kom zoo raws sijhawm.	Cov txiaj ntsig tau raug cuam tshuam ua ntej muaj; Cov kws kho mob tsuas yog pib kho cov tshuaj tua kab mob tam sim no. Tus neeg mob tau txais kev kho mob empirical rau 2-3 hnub.