Cov PCR (dPCR) yog cov txheej txheem uas faib cov txheej txheem PCR cov tshuaj tiv thaiv rau hauv ntau cov tshuaj tiv thaiv me me (xws li cov tee dej, microchambers, thiab lwm yam), kom ntseeg tau tias txhua chav muaj tsuas yog 0, 1 lossis ob peb lub hom phiaj molecules. Tom qab ntawd, txhua chav nyob ntawm nws tus kheej nthuav dav thiab suav cov lej zoo, yog li ua tiav cov txiaj ntsig ntawm lub hom phiaj nucleic acid molecules.
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FAQ
Q: Dab tsi yog qhov zoo ntawm digital PCR piv rau qPCR?
A: Cov txiaj ntsig tseem ceeb ntawm Digital PCR dhau qPCR
Tsib Qhov Zoo Tshaj Plaws ntawm Digital PCR
Cov txiaj ntsig zoo tag nrho yog los ntawm nws cov hauv paus ntsiab lus ntawm ""partitioning"" thiab ""endpoint kiag li suav."
1. Cov Quantification yam tsis muaj Standard Curve
2. Ultra-High Sensitivity thiab Rare Variant Detection
3. Superior Precision thiab Reproducibility
4. Ua siab ntev rau PCR Inhibitors
5. Kev ywj pheej ntawm Amplification Efficiency
Q: Digital PCR, qPCR, thiab kab lis kev cai ntawm cov ntshav rau kev sib piv hauv kev kuaj ntshav cov kab mob
A:
| Feature | Digital PCR | qPCR | Ntshav kab lis kev cai |
| Txoj Cai Core | Qhov kawg ntawm qhov tseeb nucleic acid suav | Tiag -lub sij hawm txheeb ze nucleic acid kom muaj nuj nqis | Kev loj hlob microbial thiab cog qoob loo |
| Kev Ntsuas Lub Hom Phiaj | Kab mob- tshwj xeeb nucleic acids (DNA/RNA) | Kab mob- tshwj xeeb nucleic acids (DNA/RNA) | Siv tau, culturable microorganisms |
| Lub sijhawm hloov pauv | Ceev ceev (Peb TAT 3 teev) | Ceev ceev Tab sis Filmarray BCID2 yog raws li cov ntshav zoo |
qeeb (2-5 hnub, avg. 24-48 teev) |
| rhiab heev | Tshwj xeeb siab (ib -theem luam) | Siab | Lower (requires >10-100 CFU / ml) |
| Quantification | Absolute (cov ntawv luam / mL) | kwv tij (Ct tus nqi, xav tau tus qauv nkhaus) | Semi-quantitative (lub sij hawm-to-positivity, colony suav) |
| Cov nyhuv ntawm cov tshuaj tua kab mob | Tsis muaj teebmeem (ntaus nucleic acid los ntawm cov kab mob tuag / nyob) | Tsis muaj teebmeem (ntaus nucleic acid los ntawm cov kab mob tuag / nyob) | Kev cuam tshuam hnyav (cov tshuaj tua kab mob ua ntej tuaj yeem ua rau muaj qhov tsis zoo) |
| Inhibitor kam rau ua | Zoo heev, ua siab ntev rau cov ntshav inhibitors | Tsis muaj zog, raug rau cov ntshav inhibitors | N / A, tab sis raug rau microbial inhibition |
| Pathogen Coverage | Lub hom phiaj, yuav tsum tau paub ua ntu zus | Lub hom phiaj, yuav tsum tau paub ua ntu zus | Dav -spectrum, tsis ncaj ncees, tuaj yeem ntes cov kab mob tsis paub / tsis tshua muaj |
| Tus nqi kho mob tseem ceeb | Thaum ntxov, meej, ntau qhov kev kuaj mob | Cov cuab yeej siv no feem ntau siv rau hauv cov xwm txheej raws li kev kuaj ntshav zoo. | Kev kuaj pom tseeb & kev qhia kho mob (Gold Standard, Slow) |
Raws li ib qho ntawm cov ua lag luam digital PCR thiab cov muag khoom nyob rau hauv Suav teb, peb muab ntau yam khoom zoo tshaj plaws. Thov koj xav tias dawb rau cov lag luam wholesale kev cai ua digital PCR los ntawm peb lub hoobkas. Tiv tauj peb kom paub meej ntxiv.
